Leucine ABA,Ile回复突变 (L亮氨酸) Brevibacterium fla 28.C L-lysine Brevibacterium flavum Thrs Met 25.0 (L赖氨酸) Brevibacterium flavum AEC 32.0 Brevibacterium fayum AEc Ala CCL mLr 60.0 Brevibacterium flavum AEC,AHV 29.0 Candida pelliculosa AECr C glutamicum AEC Homoser. Leu. Pant 42.0 L-Methionin C glutamicum Thr. ETH. SME. MethX 2.0 L蛋氨酸) L-Phenylalanine Brevibacterium flavum MFPr 2.2 (L苯丙氨酸)| Bacillus subtillis SFPr C glutamicum PFPT PAPD B lactofermentum 5MT, PFP Decs, Tyr, Met 24.8 L-Serine C. gha glutamicum OMS MSE ISE 3.8 (L丝氨酸) L-Threonine Escherichia coli AHV Met lle 6.1 Brevibacterium flavum AHV Met 18.0 (L苏氨酸) Ahve Met aecr Brevibacterium flavum AEC. AHV Serratia marcescens Ile DAP AHVr aecr Thr dehydrogenase L-Tryptophan Brevibacterium flavum 5FTMFPPhe 6.2 (L色氨酸) Brevibacterium flavum 5FT AZS PFP Tyr C glutamicum SMT 4MT 6FT TrpHX PFP PAP TyrHX Phe, PhehX Tyr L-Tyrosine Brevibacterium flavum MFPr (L酪氨酸) 3AT, PAP PFP, TyrHX, Phe 176 L-Valine Brevibacterium flavum TA 31.0 L-缬氨酸) 注:ABA一α-氨基丁酸:AEC一S-(β氨乙酰基)胱氨酸:AZ一氮鸟嘌呤;AHV一a-氨 基β-羟基戊酸:AT一氨基-酪氨酸;AU一氮鸟嘧啶;AZL一氮亮氨酸;AZS一氮丝氨酸 CCL一α氯代己内酰胺;Dec德夸霉素;DAP一a,ε-二氨基庚二酸;ETH-乙硫氨酸: FT一氟代色氨酸;HUR一羟基尿苷:HX一氧污酸;ISE一异丝氨酸:MFP-m-氟代苯丙氨 酸:MG一巯基鸟嘌呤:MH一甲基组氨酸;M-γ-甲基赖氨酸:MP一巯基嘌呤:MSE γ-甲基丝氨酸:M一甲基色氨酸:OMS—0-甲基丝氨酸:OMI-0-甲基苏氨酸:PAP p甲基苯丙氨酸;PFP一p氟代苯丙氨酸;SME一硒蛋氨酸;TA一噻脞丙氨酸;TL一硫代 异亮氨酸;诹RA-1,2,4三叠氮丙氨酸;TU一硫代鸟嘧啶;Arg(F)一缺失精氨酸反馈抑制; Arg(Rr)一缺失精氨酸操纵子的遏制;Arg(D)一缺失精氨酸降解酶 L-苏氨酸的生产菌选育也采用营养缺陷一调节突变的方法。如前所述,L-苏氨酸的积累 受到L-苏氨酸和L-赖氨酸对高丝氨酸脱氢酶的协同抑制作用,但是采用异亮氨酸营养缺9 L-Leucine (L-亮氨酸) Serratia marcescens Brevibacterium flavum ABAr , Ile-回复突变 TAr , Met－ , Ile－ 13.5 28.0 L-Lysine (L-赖氨酸) Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Candida pelliculosa C. glutamicum Thrs , Mets AECr AECr , Ala－ , CCLr , MLr AECr , AHVr AECr AECr , Homoser－ , Leu－ , Pant 25.0 32.0 60.0 29.0 3.2 42.0 L-Methionine (L-蛋氨酸) C. glutamicum Thr－ , ETHr , SMEr , MetHXr 2.0 L-Phenylalanine (L-苯丙氨酸) Brevibacterium flavum Bacillus subtilis C. glutamicum B. lactofermentum MFPr 5FPr PFPr , PAPr 5MTr , PFPr , Decs , Tyr－ , Met－ 2.2 6.0 9.5 24.8 L-Serine (L-丝氨酸) C. glutamicum OMSr , MSEr , ISEr 3.8 L-Threonine (L-苏氨酸) Escherichia coli Brevibacterium flavum C. glutamicum Brevibacterium flavum Serratia marcescens Serratia marcescens AHVr , Met－ , Ile－ AHVr , Met－ AHVr , Met－ , AECr AECr , AHVr Ile－ , DAP－ , AHVr Ile－ , DAP－ , AHVr AECr Thr dehydrogenase－ , Thr deaminase－ , 6.1 18.0 14.0 14.8 12.7 25.0 L-Tryptophan (L-色氨酸) Brevibacterium flavum Brevibacterium flavum C. glutamicum 5FTr MFPr Phe－ 5FTr AZSr PFPr Tyr－ 5MTr 4MTr 6FTr TrpHXr PFPr PAPr TyrHXr Phe－ , PheHXr Tyr－ 6.2 10.5 12.0 L-Tyrosine (L-酪氨酸) Brevibacterium flavum C. glutamicum MFPr 3ATr , PAPr , PFPr , TyrHXr , Phe－ 1.9 17.6 L-Valine (L-缬氨酸) Brevibacterium flavum TAr 31.0 注：ABA－α-氨基丁酸；AEC－S-（β氨乙酰基）胱氨酸；AZ－氮鸟嘌呤；AHV－α-氨 基β-羟基戊酸；AT－氨基-酪氨酸；AU－氮鸟嘧啶；AZL－氮亮氨酸；AZS－氮丝氨酸； CCL－α氯代己内酰胺；Dec－德夸霉素；DAP－α，ε-二氨基庚二酸；ETH－乙硫氨酸； FT－氟代色氨酸；HUR－羟基尿苷；HX－氧污酸；ISE－异丝氨酸；MFP－m-氟代苯丙氨 酸；MG－巯基鸟嘌呤；MH－甲基组氨酸；ML－γ-甲基赖氨酸；MP－巯基嘌呤；MSE－ γ-甲基丝氨酸；MT－甲基色氨酸；OMS－Ο-甲基丝氨酸；OMT－Ο-甲基苏氨酸；PAP－ p-甲基苯丙氨酸；PFP－p-氟代苯丙氨酸；SME－硒蛋氨酸；TA－噻脞丙氨酸；TIL－硫代 异亮氨酸；TRA－1,2,4-三叠氮丙氨酸；TU－硫代鸟嘧啶；Arg(Fr)－缺失精氨酸反馈抑制； Arg(Rr)－缺失精氨酸操纵子的遏制；Arg(D)—缺失精氨酸降解酶 L-苏氨酸的生产菌选育也采用营养缺陷—调节突变的方法。如前所述, L-苏氨酸的积累 受到 L-苏氨酸和 L-赖氨酸对高丝氨酸脱氢酶的协同抑制作用，但是采用异亮氨酸营养缺