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Step 1: Denaturation变性 94°C TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGAC GATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTATGAACGTAGTAGCATAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA Step 1 : Denaturation 变性 94°C