Step2: Annealing退火 primer-dependent TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGAC GATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT GCTGCTACGTAG CGTAGTAGCA CTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGC TAAACTAGCTATGAACGTAGTAGCATAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA Step 2 : Annealing 退火 T° primer-dependent CGTAGTAGCA GCTGCTACGTAG