1. Heat-fix a smear of Bacillus megaterium as follows: a. Using the dropper bottle of distilled water found in your staining rack, place a small drop of water on a clean slide by touching the dropper to the slide. b. Aseptically remove a small amount of the culture from the edge of the growth on the agar surface and generously mix it with the drop of water until the water turns cloudy. c. Burn the remaining bacteria off of the loop. d. Using the loop, spread the suspension over the entire slide to form a thin film. e. Allow this thin suspension to completely air dry. f. Pass the slide (film-side up) through the flame of the bunsen burner 3 or 4 times to heat-fix. 四 操作步骤1. Heat-fix a smear of Bacillus megaterium as follows: a. Using the dropper bottle of distilled water found in your staining rack, place a small drop of water on a clean slide by touching the dropper to the slide. b. Aseptically remove a small amount of the culture from the edge of the growth on the agar surface and generously mix it with the drop of water until the water turns cloudy. c. Burn the remaining bacteria off of the loop. d. Using the loop, spread the suspension over the entire slide to form a thin film. e. Allow this thin suspension to completely air dry. f. Pass the slide (film-side up) through the flame of the bunsen burner 3 or 4 times to heat-fix. 四 操作步骤