Steady State Assumption E+S,包BS号E+P Steady state is defined as the state during which the enzyme-substrate complex,[ES],remains constant,or d[ES]=0 dt Pre-steady state is the state during which [ES]builds up,usually very fast; MM equation concerns the reaction rate that is measured only when the steady state has reached.Steady State Assumption • Steady state is defined as the state during which the enzyme-substrate complex, [ES], remains constant, or • Pre-steady state is the state during which [ES] builds up, usually very fast; • MM equation concerns the reaction rate that is measured only when the steady state has reached. 0 [ ] − = dt d ES E + S ES E + P k1 k2 K-2