SCHIASTOSOMA JAPONICUM Objectives and Requirements 1. To understand characteristics of life cycle and morphology of its developmental 2. To observe the pathological changes of schistosomiasis in infected animal 3. To study morphology of ova, laboratory diagnostic methods and their princi 4. To gain fundamental knowledge of infective stage, infection mode, methods and significance of immunologic tests commonly used Observation and Experiment L Adult worm (1)See the live specimen(Demonstration) (1)See the preserved specimen(Demonstration) Adults are dioecious. a male and a female live together (3)Study stained specimen(Manipulation Note the shape, size and color of both sexes, oral and ventral suckers and gynecophoral canal of male, the number, shape, size and arrangement of testes, ovary and uterus of female. Females are long and slender, the posterior half of the body is a little thick and dark brown in color owing to the vitelline gland and intestine. Near the anterior end of the body, there are two suckers. The part of the male body behind the ventral sucker is flattened, and rolled ventrally along the length to form a groove, canal is gynecophonus, into which the female worm fits What is the meaning of dioecious? (2)See the seM photograph of adult worm( Demonstration) 2. Larva and intermediate host(Demonstration) (1)See the live miracidia Note the feature of movement in water (2)Study the structure of stained specimen of miracidia. (3)See the SEM photograph of miracidia (4)Identify the intermediate host Oncomelania snail (5)See the live cercariae Note the posture of the body resting beneath water surface and its movement Study stained specimen, note the internal structures and the forked tail (6)See the SEM photograph of cercariae 3. ovu (1) Place a small drop n containing S. japonicum ova on a slide9 SCHISTOSOMA JAPONICUM Objectives and Requirements 1. To understand characteristics of life cycle and morphology of its developmental stages. 2. To observe the pathological changes of schistosomiasis in infected animal. 3. To study morphology of ova, laboratory diagnostic methods and their principle. 4. To gain fundamental knowledge of infective stage, infection mode, methods and significance of immunologic tests commonly used. Observation and Experiment l. Adult worm (1) See the live specimen (Demonstration). (1) See the preserved specimen (Demonstration). Adults are dioecious, a male and a female live together. (3) Study stained specimen (Manipulation). Note the shape, size and color of both sexes, oral and ventral suckers and gynecophoral canal of male, the number, shape, size and arrangement of testes, ovary and uterus of female. Females are long and slender, the posterior half of the body is a little thick and dark brown in color owing to the vitelline gland and intestine. Near the anterior end of the body, there are two suckers. The part of the male body behind the ventral sucker is flattened, and rolled ventrally along the length to form a groove, canalis gynecophorus, into which the female worm fits. What is the meaning of dioecious? (2) See the SEM photograph of adult worm (Demonstration). 2. Larva and intermediate host (Demonstration) (1) See the live miracidia. Note the feature of movement in water. (2) Study the structure of stained specimen of miracidia. (3) See the SEM photograph of miracidia. (4) Identify the intermediate host Oncomelania snail. (5) See the live cercariae. Note the posture of the body resting beneath water surface and its movement. Study stained specimen, note the internal structures and the forked tail. (6) See the SEM photograph of cercariae. 3. Ovum (1) Place a small drop of suspension containing S. japonicum ova on a slide