dissect them to find adult worms FASCIOLOPSIS BUSKI Objectives and requirements 1. To study the life history and feature in morphology of F buski 2. To study the laboratory diagnosis of this fluke 3. To gain some knowledge of plant vectors and the importance in prophylax Observation and experiment 1. Adult worm (1)See the preserved specimens(Demonstration) Note the shape, size, color and the positions of oral and ventral suckers and their sizes. large and thick (1)Study the stained specimen(Manipulation First with naked eye, then under low power microscope, note the adhesive organ, ceca and reproductive system of both sexes. The intestine is composed of two blind tracts without side branching, and the ventral sucker is very large 2. Larva, intermediate host and vector(demonstration) (1)See the snail intermediate host(Planorbis (2)See the cercaria (3)See the stained specimen of cyst (4)See the vector: aquatic plants(caltrop, water chestnut etc.) 3. Ovum (1)See the preserved specimens(Manipulation) Note its large size, 130-140 u mX80-85 H m, oval shape, pale yellow in color, thin shell, small operculum, an germinal cell surrounded by yolk granules Materials examined: feces (2)See the SEM photograph of ovum Exercise Draw and label F. buski egg in detail. Thinking 1. Draw a life cycle draft of f. buski8 dissect them to find adult worms. FASCIOLOPSIS BUSKI Objectives and Requirements 1. To study the life history and feature in morphology of F. buski. 2. To study the laboratory diagnosis of this fluke. 3. To gain some knowledge of plant vectors and the importance in prophylaxis. Observation and Experiment 1. Adult worm (1) See the preserved specimens (Demonstration). Note the shape, size, color and the positions of oral and ventral suckers and their sizes, large and thick. (1) Study the stained specimen (Manipulation). First with naked eye, then under low power microscope, note the adhesive organ, ceca and reproductive system of both sexes. The intestine is composed of two blind tracts without side branching, and the ventral sucker is very large. 2. Larva, intermediate host and vector (Demonstration) (1) See the snail intermediate host (Planorbis). (2) See the cercaria. (3) See the stained specimen of cyst. (4) See the vector: aquatic plants (caltrop, water chestnut etc.). 3. Ovum (1) See the preserved specimens (Manipulation). Note its large size, 130～140μm×80～85μm, oval shape, pale yellow in color, thin shell, small operculum, an germinal cell surrounded by yolk granules. Materials examined: feces. (2) See the SEM photograph of ovum. Exercise 1.Draw and label F. buski egg in detail. Thinking 1.Draw a life cycle draft of F. buski