Note the capsules and their surrounding damaged tissues B. See the infected liver( Demonstration) 2. Pagumogonimus skrjabini (1)Adult worm(Demonstration) A. See the preserved specimen B. See the stained specimen (2)Intermediate host(Demonstration) ee the first intermediate host-snail (Tricula humida) Exercise 1. Draw P. westermani egg in detail and label 2. Label the adult worm of p westermani 3. Write the result report of cyst isolation, to have an analysis of epidemiology of P. 4. Draw a life cycle draft of P westermani Thinking 1. How to explain paragonimiasis is a natural epidemic source disease in mountainous egion and forest? and what are the epidemic characteristics of paragonimiasis? R eference 1. Detection of sputum for egg of P westermani Take a clean slide, place a drop of saline in the center of it, pick up a small amount of the sputum and mix it with the drop of saline, examine under microscope (2)Egg concentration Collect the sputum of 24 h, pour into a measuring glass and add equal quantity of 10% NaoH, mix, sediment naturally for 6 h8 h, remove supernatant, take the sediment and examine under microscop 2. Survey biological hosts of P westerman in epidemiology Choose one or more brooks, capture Melania snail and stream crab or crayfish (1)Break up Melania snail and place the internal organs on a slide, open them and put a drop of 0.45% saline, find sporocyst, redia and cercaria under microscop (2)Dissect stream crab or crayfish and find encysted metacercaria 3. Infection of p westermani for dog Isolation of cysts from crayfish and mixing with meat, feed dogs, each one with 100-200 cysts according to the sizes of dogs. Kill the infected dogs 2-3 months later, open chest and expose the lungs, on which the nodular cysts can be observed7 Note the capsules and their surrounding damaged tissues. B. See the infected liver (Demonstration). 2. Pagumogonimus skrjabini (1) Adult worm (Demonstration). A. See the preserved specimen. B. See the stained specimen. (2) Intermediate host (Demonstration) See the first intermediate host-snail (Tricula humida). Exercise 1. Draw P. westermani egg in detail and label. 2. Label the adult worm of P. westermani. 3. Write the result report of cyst isolation, to have an analysis of epidemiology of P. westermani. 4. Draw a life cycle draft of P. westermani. Thinking 1. How to explain paragonimiasis is a natural epidemic source disease in mountainous region and forest? And what are the epidemic characteristics of paragonimiasis? Reference 1. Detection of sputum for egg of P. westermani (1) Sputum smear Take a clean slide, place a drop of saline in the center of it, pick up a small amount of the sputum and mix it with the drop of saline, examine under microscope. (2) Egg concentration Collect the sputum of 24 h, pour into a measuring glass and add equal quantity of 10% NaOH, mix, sediment naturally for 6 h～8 h, remove supernatant, take the sediment and examine under microscope. 2. Survey biological hosts of P. westerman in epidemiology Choose one or more brooks, capture Melania snail and stream crab or crayfish . (1) Break up Melania snail and place the internal organs on a slide, open them and put a drop of 0.45% saline, find sporocyst, redia and cercaria under microscope. (2) Dissect stream crab or crayfish and find encysted metacercaria. 3. Infection of P. Westermani for dog Isolation of cysts from crayfish and mixing with meat, feed dogs, each one with 100～200 cysts according to the sizes of dogs. Kill the infected dogs 2～3 months later, open chest and expose the lungs, on which the nodular cysts can be observed