Pstl等产生的3粘性末端 5.G-C-T-C-T-G-C-A-G-G-A-G.3' 111111111111 3.C-G-A-G-A-C-G-T-C-C-T-C.5' Pst37℃ 5.G-C-T-C-T-G-C-A-OH P-G-G-A-G.3' 1111 1111 3.C-G-A-G-P oH-A-C-G-T-C-C-T-C.5' 退火4-7℃ OH P 5.G-C-T-C-T-G-C-A G-G-A-G.3" 11111111II11 3.C-G-A-G A-C-G-T-C-C-T-C.5' P OH