EcoRI等产生的5粘性末端 5.G-C-T-G-A-A-T-T-C-G-A-G.3' 111111111111 3.C-G-A-C-T-T-A-A-G-C-T-C.5' EcoRI37℃ 55.G-C-T-G-OH P-A-A-T-T-C-G-A-G.3' I111 IIII 3.C-G-A-C-T-T-A-A-P OH-G-C-T-C.5' 退火4-7℃ ©HP 55.G-C-T-G A-A-T-T-C-G-A-G.3' 11111111111i 35.C-G-A-C-T-T-A-A G-C-T-C.5' P⊙H