Figure 2.3 I.Preparing a smear Steps of Spread culture in thin Dry in air film over slide staining Il.Heat fixing and staining cells for microscopic observation Pass slide through Flood slide with stain; flame to heat fix rinse and dry Ill.Microscopy 100× Slide Oil Place drop of oil on slide; examine with 100x objective lensFigure 2.3 I. Preparing a smear II. Heat fixing and staining III. Microscopy Spread culture in thin film over slide Pass slide through flame to heat fix Dry in air Flood slide with stain; rinse and dry Place drop of oil on slide; examine with 100 objective lens Slide Oil Steps of staining cells for microscopic observation