(4)菌落杂交(colony hybr idization) Master plate Nitrocellulose filter Phage plaque Place nitrocellulose filter on plate Phages transferred to filter Denature, Hybridize with probe Perform autoradiography 该法可从大量细菌中筛选含特异的DNA序列及基因工程重组体。15 （4）菌落杂交（colony hybridization） 该法可从大量细菌中筛选含特异的DNA序列及基因工程重组体