Vol.29 Suppl 2 邢奕等：应用耐冷菌株改善寒冷地区冬季人工湿地系统生物脱氨效果 57。 35 定期.而NL02则需要6天. 301 (2)在pH值为7一8时，各菌株生长得最好 25 放线菌表现为嗜弱酸性，细菌和霉菌则嗜弱碱性. 20 生物荧光层析光谱实验发现三种菌株都在15℃左 1,25% 右活性最大，0℃以下仍有一定的活性，高于35℃ ·2.50% 时，基本失去正常的代谢能力 -5.00% -10.00% (3)当实验菌液投加量为5.0%，温度为6℃ 00 时，三种耐冷菌对氨氮的去除率分别为57.7%、 2345678910 时间d 59.0%及58.7%.相同条件下，投加混合菌种可使 氨氯的去除率提高到67.2%. 图8氨氨随不同N03投加量变化曲线 随着耐冷机制的进一步研究以及耐冷菌在人工 湿地系统中投加实验的深入，必将推动解决冬季低 温条件下人工湿地脱氮效果差等问题的进程，进而 为人工湿地工艺在我国北方地区的推广扫清障碍. 25 参考文献 15 -1.25% 【刂王孝纲.日本下水污泥的资源化现状与发展方向衣业环境 10 -2.50% 一5.00% 与发展，1998,15(2)：42 5 ←一10.00% 【习成水平，吴振斌，况琪军.人工湿地植物研究湖泊科学， 2002,142):179 0 4567 89 时间d [3 Meutia A A.Treatment of laboratory wastew aterin aconstructed wetland comparing surface and subsurface flow.Water Sci Tech- 图9氨氨随不同NL04投加量变化曲线 ndl,2001,4411-12):499 【4张希衡。废水厌氧生物处理工程.北京：中国环境科学出版 培养出三种菌株，生物荧光层析光谱实验，证实所培 社.1996 养的三种菌株属于耐冷菌属，环境温度为6℃时， [习韩晓云，姜安玺，何丽蓉.低温菌及其在环境工程中的应用 NL01在第4天进入稳定期，NL03在第5天进入稳 东北林业大学学报2003.31(2)：33 Application of psychrotrophses in improving bio-treatment efficiency of nitrogen in constructed wetland in cold area during winter XING Yi,QIAN Dayi,YING Gaoxiang 1)Civil Environment Engineering School.University of Science and Techmology Beijing Beijing 100083,Chima 2)Environmental Engineering Sciences,University of Florida,Gainesville,FL32611.USA ABSTRACT Based on probing experiments,three psy chrotrophses culture mediums were discovered and uti- lized to culture bacilli,actinomyces,and mildew,respectively.Cultured pschrotrophses were separated and pu- rified at 6 C.The physio-characteristics of psychrotrophses were identified and their nitration abilities were in- spected by considering effects of pH and temperature.A best grow th of psychrotrophses was detected when the pH value was in the range of 7 to 8.Biology fluorescence chromatography spectrum test indicates that all psy- chrotrophses present the maximal activity at 15C,still have definite activity at 0C and lose natural metaboliz- ing ability at above 35 C.Under the conditions of 6Cand psychrotrophses dosage of 5.0%,the ammonia-ni- trogen removal efficiencies of the three psychrotrophses are 57.7%,59.0%,and 58.7%respectively.If a mix- ture of psychrotrophses is applied under the same condition,the ammonia-nitrogen removal efficiency increases to67.2%. KEY WORDS sew age reuse;constructed wetland;low temperature;nitrogen removal;psychrotrophses图 8 氨氮随不同 NL03 投加量变化曲线 图 9 氨氮随不同 NL04 投加量变化曲线 培养出三种菌株, 生物荧光层析光谱实验, 证实所培 养的三种菌株属于耐冷菌属, 环境温度为 6 ℃时, N L01 在第 4 天进入稳定期, NL03 在第 5 天进入稳 定期, 而 N L02 则需要 6 天. ( 2) 在 pH 值为 7 ～ 8 时, 各菌株生长得最好. 放线菌表现为嗜弱酸性, 细菌和霉菌则嗜弱碱性. 生物荧光层析光谱实验发现三种菌株都在 15 ℃左 右活性最大, 0 ℃以下仍有一定的活性, 高于 35 ℃ 时, 基本失去正常的代谢能力. ( 3) 当实验菌液投加量为 5.0 %, 温度为 6 ℃ 时,三种耐冷菌对氨氮的去除率分别为 57.7 %、 59.0 %及 58.7 %.相同条件下, 投加混合菌种可使 氨氮的去除率提高到 67.2 %. 随着耐冷机制的进一步研究以及耐冷菌在人工 湿地系统中投加实验的深入, 必将推动解决冬季低 温条件下人工湿地脱氮效果差等问题的进程, 进而 为人工湿地工艺在我国北方地区的推广扫清障碍. 参 考 文 献 [ 1] 王孝纲.日本下水污泥的资源化现状与发展方向.农业环境 与发展, 1998, 15(2) :42 [ 2] 成水平, 吴振斌, 况琪军.人工湿地植物研究.湖泊科学, 2002, 14( 2) :179 [ 3] Meutia A A .T reatment of laborat ory w astew ater in a constructed wetland comparing surf ace and subsurf ace flow .Water Sci Tech￾nol, 2001, 44( 11 ～ 12) :499 [ 4] 张希衡.废水厌氧生物处理工程.北京:中国环境科学出版 社, 1996 [ 5] 韩晓云, 姜安玺, 何丽蓉.低温菌及其在环境工程中的应用. 东北林业大学学报, 2003, 31( 2) :33 Application of psychrotrophses in improving bio-treatment efficiency of nitrogen in constructed wetland in cold area during winter X ING Yi 1) , QIAN Dayi 1) , YING Gaox iang 2) 1) Civil &Environment Engineering S chool, University of Science and Technology Beijing, Beijing 100083, C hina 2) Environmental Engineering S ciences, University of Florida, Gainesville, FL 32611, USA ABSTRACT Based on probing experiments, three psy chro trophses culture mediums were discovered and uti￾lized to culture bacilli, actinomyces, and mildew , respectively .Cultured pschro trophses were separated and pu￾rified at 6 ℃.The physio-characteristics of psychrotrophses w ere identified and their nitration abilities were in￾spected by considering effects of pH and temperature.A best g row th of psychro trophses w as detected when the pH value w as in the range of 7 to 8 .Biology fluorescence chromatog raphy spectrum test indicates that all psy￾chrotrophses present the maximal activity at 15 ℃, still have definite activity at 0 ℃, and lose natural metaboliz￾ing ability at above 35 ℃.Under the conditions of 6 ℃and psychro trophses dosage of 5.0 %, the ammonia-ni￾trogen removal efficiencies of the three psychrotrophses are 57.7 %, 59.0 %, and 58.7 %respectively .If a mix￾ture of psychro trophses is applied under the same condition, the ammonia-nitrogen removal efficiency increases to 67.2 %. KEY WORDS sew age reuse;constructed w etland ;low temperature ;nitrogen removal ;psychro trophses Vol.29 Suppl.2 邢奕等:应用耐冷菌株改善寒冷地区冬季人工湿地系统生物脱氮效果 · 57 ·