Content- Column Chromatography 802 Packing column: Wet packing technology is selected in this experiment Elution: Pressurized rapid elution is selected in this experiment Packing column: 1. 15 g Silica gel and 60 mL PE are placed in a 100 ml beaker, and sitred evenly 2. Add 10 mL PE to the fixed column, and keep it dripping naturally 3. Transfer the suspension pe and silica gel to the column. Compact the stationar phase, and ensure that there are no bubbles in the column 4. Add 1-2 mm quartz sand on the surface of silica gel Add sample A l ml sample is evenly dropped onto the surface of the quartz sand and allow the crude product to adhere to the silica gel under light pressure with the assistance of the eluent pe Elution: Under the conditions of pressurized elution, the Pe/ea(10/1)eluent is used firstly. After the first compound (ribbon)is completely eluted, PE/EA(/1)0,) eluent is used. Each compound is collected in a separate conical flask. JContent – Column Chromatography Packing column: 1. 15 g Silica gel and 60 mL PE are placed in a 100 ml beaker, and sitred evenly. 2. Add 10 mL PE to the fixed column, and keep it dripping naturally. 3. Transfer the suspension PE and silica gel to the column. Compact the stationar. phase, and ensure that there are no bubbles in the column. 4. Add 1-2 mm quartz sand on the surface of silica gel. Add sample: A 1 mL sample is evenly dropped onto the surface of the quartz sand and allow the crude product to adhere to the silica gel under light pressure with the assistance of the eluent PE. Elution: Under the conditions of pressurized elution, the PE/EA (10/1) eluent is used firstly. After the first compound (ribbon) is completely eluted, PE/EA (2/1) eluent is used. Each compound is collected in a separate conical flask. Packing column：Wet packing technology is selected in this experiment Elution：Pressurized rapid elution is selected in this experiment