月 Notes 1. During the packing process, it is required that the stationary phase is uniform and there are no bubbles inside, otherwise the separation efficiency will be affected 2. The stationary phase is soaked by the eluent without cracking, otherwise the separation efficiency will be affected 3. Before loading the sample, a small amount of quartz sand is added on the surface of silica gel to avoid smashing the stationary phase when dropping the sample 4. Weigh the silica gel in a fume cupboard to prevent silica gel from inhaling into the nasal cavity 5. During the elution process, the eluent should be poured slowly to avoid smashing the stationary phase 6. During the elution process, ensuring the appropriate pressure and flow rate of the eluent are critical 》Notes 1. During the packing process, it is required that the stationary phase is uniform and there are no bubbles inside, otherwise the separation efficiency will be affected. 2. The stationary phase is soaked by the eluent without cracking, otherwise the separation efficiency will be affected。 3. Before loading the sample, a small amount of quartz sand is added on the surface of silica gel to avoid smashing the stationary phase when dropping the sample. 4. Weigh the silica gel in a fume cupboard to prevent silica gel from inhaling into the nasal cavity. 5. During the elution process, the eluent should be poured slowly to avoid smashing the stationary phase. 6. During the elution process, ensuring the appropriate pressure and flow rate of the eluent are critical