antibody preparation distinguishes the three different antigens, which form independent precipitin arcs. In reaction(c) the antigens share epitope l but one antigen also has epitope 2 This is the same situation as in(a), but in this case the antibody can distinguish them, by virtue of being able to react against both epitopes. a line of identity forms with anti-epitope with the addition of a'spur'where the anti-epitope 2 has reacted with the second epitope, thus indicating partial rather than total identity between the antigen preparations. 图85免疫电泳法 immunoelectrophoresis 1. separation of antigens 3. diffusion and precipitation 2. antiserum in trough trough Fig 8-5 Immunoelectrophoresis allows the comparison of complicated mixtures antigen such as are found in serum. 1. Antigens are separated in an agar gel by placing an electric charge across it. The gels pH is chosen so that positively charged proteins move to the negative electrode and negatively charged proteins to the positive. 2. a trough is then cut between the wells and filed with the antibody, which is left to diffuse. 3. The antigens and antibody form precipitin arcs. 免疫电泳法( immunoelectrophoresis)(见图85)是在凝胶介质中将电泳法与扩散法相 结合的一种免疫化学方法,用以研究抗原和抗体。免疫电泳是使血清在琼脂或琼脂糖中进行 的电泳。在一定电场强度下,由于血清中各种免疫球蛋白的分子大小以及荷电状态和荷电量 均有差异,因而它们的泳动速率也各不相同,加上电泳过程中电渗作用的影响,使各自组分 得到分离163 antibody preparation distinguishes the three different antigens, which form independent precipitin arcs. In reaction(c) the antigens share epitope 1 but one antigen also has epitope 2. This is the same situation as in (a), but in this case the antibody can distinguish them, by virtue of being able to react against both epitopes. A line of identity forms with anti-epitope 1, with the addition of a ‘spur’ where the anti-epitope 2 has reacted with the second epitope, thus indicating partial rather than total identity between the antigen preparations. 图 8-5 免疫电泳法 Fig 8-5 Immunoelectrophoresis allows the comparison of complicated mixtures of antigen such as are found in serum. 1. Antigens are separated in an agar gel by placing an electric charge across it. The gel’s pH is chosen so that positively charged proteins move to the negative electrode and negatively charged proteins to the positive. 2. A trough is then cut between the wells and filed with the antibody, which is left to diffuse. 3. The antigens and antibody form precipitin arcs. 免疫电泳法（immunoelectrophoresis）（见图 8-5）是在凝胶介质中将电泳法与扩散法相 结合的一种免疫化学方法，用以研究抗原和抗体。免疫电泳是使血清在琼脂或琼脂糖中进行 的电泳。在一定电场强度下，由于血清中各种免疫球蛋白的分子大小以及荷电状态和荷电量 均有差异，因而它们的泳动速率也各不相同，加上电泳过程中电渗作用的影响，使各自组分 得到分离