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在一定电场强度下,抗原与相应抗体在琼脂介质中加速扩散相遇而形成复合物沉淀,这 种检测方法称作电免疫扩散法( electroimmunodiffusion)。由于操作方法不同,电免疫扩散 法可分为对流免疫电泳( countercurrent immunoelectrophoresis)(见图8-6),交叉免疫电泳 crossed immunoelectrophoresis)和火箭免疫电泳( rocket immunoelectrophoresis)(见图 8-7) 图8-6(上图)对流免疫扩散法 图8-7(下图)火箭免疫电泳法 countercurrent electrophoresis agar pH 8.0 rocket electrophoresis precipitin rockets A④A④A Fig 8-6, 8-7 Countercurrent electrophoresis is performed in agar gels where the ph is hosen so that the antibody is positively charged and the antigen being tested is negatively charged. By applying a voltage across the gel the antigen and antibody move towards each164 在一定电场强度下,抗原与相应抗体在琼脂介质中加速扩散相遇而形成复合物沉淀,这 种检测方法称作电免疫扩散法(electroimmunodiffusion)。由于操作方法不同,电免疫扩散 法可分为对流免疫电泳(countercurrent immunoelectrophoresis)(见图 8-6),交叉免疫电泳 (crossed immunoelectrophoresis)和火箭免疫电泳(rocket immunoelectrophoresis)(见图 8-7)。 图 8-6(上图) 对流免疫扩散法 图 8-7(下图) 火箭免疫电泳法 Fig 8-6, 8-7 Countercurrent electrophoresis is performed in agar gels where the pH is chosen so that the antibody is positively charged and the antigen being tested is negatively charged. By applying a voltage across the gel the antigen and antibody move towards each
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