Partition Chromatography: ·Most popular method Low molecular weight(mw<3000)analytes ·Polar or non-polar Bonded stationary phase column (liquid chemically bonded to support particles) 3,5 or 10 um hydrolyzed silica particles coated with siloxanes R2 Normal phase HPLC nonpolar solvent/polar column Reversed phase HPLC polar solvent/nonpolar column CEM 333 page 16.9Partition Chromatography: • Most popular method • Low molecular weight (mw<3000) analytes • Polar or non-polar • Bonded stationary phase column (liquid chemically bonded to support particles) 3, 5 or 10 mm hydrolyzed silica particles coated with siloxanes Si O * R1 R2 n Normal phase HPLC nonpolar solvent/polar column Reversed phase HPLC polar solvent/nonpolar column CEM 333 page 16.9