BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 o Promoting remodeling/cell migration through synthetic networks PEG PEG HcHo. 定长 B K. Mann. AS. Gobin. A.T. Tsai Schmedlen. J. L West. Biomaterials 22 Example synthesis strategy: photoencapsulation of live cells Photoencapsulation: expose solution of cells, prepolymer/cross-linkermonomer, and photoinitiator to light to initiate free radical polymerization In sterile culture media Cyclohexyl phenyl ketone Provides very rapid polymerization(2-20 seconds typical), at neutral pH and room temp. -37C soft UV photoinitiators are common and non-toxic(illuminate at 365 nm) Lecture 7-Hydrogels 1 8of15BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 o Promoting remodeling/cell migration through synthetic networks: PEG photopolymerization = = PEG peptides -GWGLGPAGK- -CH2CH2O￾collagenase sequence collagenase B.K. Mann, A.S. Gobin, A.T. Tsai, R.H. Schmedlen, J.L. West, Biomaterials 22, 3045 (2001) Example synthesis strategy: photoencapsulation of live cells5 • Photoencapsulation: expose solution of cells, prepolymer/cross-linker/monomer, and photoinitiator to light to initiate free radical polymerization = = = = = = = = = = = = = = = = In sterile culture media: hν Cyclohexyl phenyl ketone: UV hν • Provides very rapid polymerization (2-20 seconds typical), at neutral pH and room temp. – 37°C • ‘soft’ UV photoinitiators are common and non-toxic (illuminate at 365 nm) Lecture 7 – Hydrogels 1 8 of 15