steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价Evaluation: The teacher will evaluate the experimental procedure done by the students on-site.The students will finish the experimental report.The students should summarize the experimental result and attach necessary documents from their lab experiment.The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 3 The Lowry Assay for Protein 1.教学目标Aims: (1)Understand the basic methods and the principle of the determination of protein content in the samples. (2)Familiar with Folin phenol reagent method principle and the method for determination of protein content. (3)Grasp the principle and method of Folin-phenol reagent to evaluate protein content. 2.教学内容Content: The Lowry method relies on two different reactions.The first is the formation of a copper ion complex with amide bonds,forming reduced copper in alkaline solutions.This is called a "Biuret"chromophore.The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate)by tyrosine and tryptophan residues.The reduced Folin-Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500-750 nm.The Biuret reaction itself is not all that sensitive.Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. 2.教学重难点Key points: Draw standard curve:Using the protein content of each standard tube as X axis, OD value as Y axis,Draw the standard curve.The standard curve can be used to determine the protein content of specimen. 4.教学方法Teaching method: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class.The teacher should display the basic experimentalsteps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价 Evaluation： The teacher will evaluate the experimental procedure done by the students on-site. The students will finish the experimental report. The students should summarize the experimental result and attach necessary documents from their lab experiment. The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 3 The Lowry Assay for Protein 1. 教学目标 Aims: （1） Understand the basic methods and the principle of the determination of protein content in the samples. （2） Familiar with Folin phenol reagent method principle and the method for determination of protein content. （3） Grasp the principle and method of Folin-phenol reagent to evaluate protein content. 2. 教学内容 Content: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. This is called a "Biuret" chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin-Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500-750 nm. The Biuret reaction itself is not all that sensitive. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. 2.教学重难点 Key points: Draw standard curve：Using the protein content of each standard tube as X axis, OD value as Y axis, Draw the standard curve. The standard curve can be used to determine the protein content of specimen. 4.教学方法 Teaching method: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class. The teacher should display the basic experimental