The teacher will evaluate the experimental procedure done by the students on-site.The students will finish the experimental report.Student should summarize the experimental result and attach necessary documents from their lab experiment. The students should also discuss the reason for experimental failures.The teacher will evaluate the report according to their experimental result and discussion. The students need to know the rules to choose and use glass pipette;how to measure the concentration of unknown solution using the standard curve method;how to use a centrifuge Experiment 2 Effects of some factors on enzymatic activity (a-salivary amylase) 1.教学目标Aims: (1)To understand the properties of the enzyme and the effects of some factors such as temperature and pH on enzymatic activity. (2)Be familiar with and deepen the understanding of the chemical nature of enzymes. (3)Grasp the saliva amylase in different temperatures,pH value,under the condition of the vitality of hydrolyzed starch size,and the chloride ion, copper ion on its enzymatic activity. 2.教学重难点Key points: Compare tubes in groups:1,2,3 to determine how the temperature influence enzymatic activity:3,4,5 to understand how pH affects enzymatic activity;3,6,7,8 to understand how different ions affect a-salivary amylase activity. 3.教学内容Content: All enzyme-catalyzed reactions are affected by temperature.The higher the temperature,the higher the reaction rate.However,enzymes are proteins that become denatured at high temperature.So each enzyme has an optimum temperature at which it operates at maximal efficiency.An enzyme's optimum temperature is usually close to the normal temperature of the organism it comes from(around 37C )Changes in pH also affect enzymes through alteration of the tertiary structure of the enzyme or active site.Furthermore,activators and inhibitors can play a key role in regulation of enzymatic activity. 4.教学方法Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class.The teacher should display the basic experimentalThe teacher will evaluate the experimental procedure done by the students on-site. The students will finish the experimental report. Student should summarize the experimental result and attach necessary documents from their lab experiment. The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. The students need to know the rules to choose and use glass pipette; how to measure the concentration of unknown solution using the standard curve method; how to use a centrifuge Experiment 2 Effects of some factors on enzymatic activity (salivary amylase) 1. 教学目标 Aims: （1） To understand the properties of the enzyme and the effects of some factors such as temperature and pH on enzymatic activity. （2） Be familiar with and deepen the understanding of the chemical nature of enzymes. （3） Grasp the saliva amylase in different temperatures, pH value, under the condition of the vitality of hydrolyzed starch size, and the chloride ion, copper ion on its enzymatic activity. 2.教学重难点 Key points: Compare tubes in groups: 1,2,3 to determine how the temperature influence enzymatic activity; 3,4,5 to understand how pH affects enzymatic activity; 3,6,7,8 to understand how different ions affect a-salivary amylase activity. 3. 教学内容 Content: All enzyme-catalyzed reactions are affected by temperature. The higher the temperature, the higher the reaction rate. However, enzymes are proteins that become denatured at high temperature. So each enzyme has an optimum temperature at which it operates at maximal efficiency. An enzyme’s optimum temperature is usually close to the normal temperature of the organism it comes from(around 37℃ ). Changes in pH also affect enzymes through alteration of the tertiary structure of the enzyme or active site. Furthermore, activators and inhibitors can play a key role in regulation of enzymatic activity. 4.教学方法 Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class. The teacher should display the basic experimental