Day 2 Examine the plates and determine the efficiency of transformation Transformation ratio Sum f converter -number of colonies x multiple of dilution X volume of transformation reaction / volume of bacterium transfer to plate Sum of competent cell colonies on control 2 X multiple of dilution X total volume of bacterium/volume of bacterium transfer to plate Transformation ratio of competent cell- Sum of Converter/sum of competent cell15 Day 2 Examine the plates and determine the efficiency of transformation. Transformation ratio: Sum of converter =number of colonies × multiple of dilution × volume of transformation reaction /volume of bacterium transfer to plate Sum of competent cell= colonies on control 2 × multiple of dilution × total volume of bacterium/volume of bacterium transfer to plate Transformation ratio of competent cell= Sum of Converter/ sum of competent cell