Basic procedure for plasmid transformation of e coli dd gentl lul plasmid competent cells lul h,o(control) 2. on ice.30 minutes 3. 42C water bath heat shock 90 seconds 4. on ice.2 minutes 5.add800μ I of le,37℃ shaker,30 minutes 6. spread 200 ul competent cells on LB/Amp plate 7. spread 200 ul control on LB/Amp or LB plate 8. room temperature until the liquid has been absorbed 9. invert the plates and incubate at 37C14 2. on ice ,30 minutes 3. 42 ℃ water bath heat shock, 90 seconds 4. on ice ,2 minutes 5. add 800 µl of LB, 37℃ shaker ,30 minutes 6. spread 200 ul competent cells on LB/Amp plate 7. spread 200 ul control on LB/Amp or LB plate 8. room temperature until the liquid has been absorbed 9. invert the plates and incubate at 37℃ competent cells 1ul Plasmid 1ul H2O(control ) 1. add gently Basic procedure for plasmid transformation of E. coli