Time required: ransformation: about I hour Day 1) Growth: approximately 16 hours for visible colonies(Day 2 Control Transfer same volume h,o to competent cells Spread on LB/Amp plate or lB plate13 Time required: Transformation: about 1 hour (Day 1 ) Growth: approximately 16 hours for visible colonies (Day 2 ) Control Transfer same volume H2O to competent cells Spread on LB/Amp plate or LB plate