defined the number of targets connected to it, which indicated the importance of the node in the network Pathway enrichment analysis for selected targets. The Database for Annotation VisualizationandIntegratedDiscovery(david,https://david.ncifcrf.gov/home.isp version 6. 7)was used for GO enrichment analysis Vaccine, adjuvant and antibodies. The hepatitis B vaccine(consisting of HBsAg and aluminium adjuvant)and HBsAg were a generous gift from the Dalian Hisser BioPharm Co, Ltd (Dalian, China). Cordycepin was purchased from Beijing Century Bioko Bio-technology Co. (Beijing, China). The endotoxin levels of these reagents were <0. 1 EU/ml as determined by an endotoxin detection assay (Tachypleus Amebocyte Lysate, Xiamen, China). Fluorescein isothiocyanate(FITC)-conjugated anti-mouse CD3, phycoerythrin (PE)-conjugated anti-mouse PE/Cy5-conjugated anti-mouse CD8a, FITC-conjugated anti-mouse CD80 FITC-conjugated anti-mouse CD86 and PE-conjugated anti-mouse CD19 antibodies were all purchased from BioLegend (BioLegend, San Diego, CA, USA) Mouse immunization and sample collection. Female BaLB/c mice at 4-6 weeks of age were obtained from the Shanghai SLAC Laboratory Animal Center and assigned to one of five experimental groups with ten mice in each gr They were maintained under specific-pathogen-free conditions at Fudan University. The principles of laboratory animal care were followed precisely. All procedures were conducted according to the guidelines established by the National Institutes of Health, and every effort was made to minimize discomfort and suffering. This study was13 defined the number of targets connected to it, which indicated the importance of the node in the network. Pathway enrichment analysis for selected targets. The Database for Annotation, Visualization and Integrated Discovery (DAVID, https://david.ncifcrf.gov/home.jsp, version 6.7) was used for GO enrichment analysis. Vaccine, adjuvant and antibodies. The hepatitis B vaccine (consisting of HBsAg and aluminium adjuvant) and HBsAg were a generous gift from the Dalian Hissen BioPharm Co., Ltd. (Dalian, China). Cordycepin was purchased from Beijing Century Bioko Bio-technology Co. (Beijing, China). The endotoxin levels of these reagents were <0.1 EU/ml as determined by an endotoxin detection assay (Tachypleus Amebocyte Lysate, Xiamen, China). Fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD3, phycoerythrin (PE)-conjugated anti-mouse CD4, PE/Cy5-conjugated anti-mouse CD8a, FITC-conjugated anti-mouse CD80, FITC-conjugated anti-mouse CD86 and PE-conjugated anti-mouse CD19 antibodies were all purchased from BioLegend (BioLegend, San Diego, CA, USA). Mouse immunization and sample collection. Female BALB/c mice at 4-6 weeks of age were obtained from the Shanghai SLAC Laboratory Animal Center and assigned to one of five experimental groups with ten mice in each group. They were maintained under specific-pathogen-free conditions at Fudan University. The principles of laboratory animal care were followed precisely. All procedures were conducted according to the guidelines established by the National Institutes of Health, and every effort was made to minimize discomfort and suffering. This study was