DNA Shuffling的内涵 What is DNA shuffling? crossovers, Darwinian Evolution deletions, Directed Evolution Natural selection of insertions, Targeted selection existing mutations ￥inversions, of created mutations 多 point mutations What happens after DNA shuffling Generation of a large library of novel genes (chimeras) Selection for improved/desired bio-functions What happens after DNA shuffling ➢ Generation of a large library of novel genes (chimeras) ➢ Selection for improved/desired bio-functions ❖ crossovers, ❖ deletions, ❖ insertions, ❖ inversions, ❖ point mutations Darwinian Evolution Natural selection of existing mutations Directed Evolution Targeted selection of created mutations What is DNA shuffling? DNA Shuffling的内涵