Problems mRNA produced must be efficiently translated and there are problems with this always happening · Bacterial ribosome binding sites are not present in eukaryotic genomes Differences in codon usage between organisms · Eukaryotic genes containing introns will not be expressed properly in prokaryotes Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology Problems mRNA produced must be efficiently translated and there are problems with this always happening • Bacterial ribosome binding sites are not present in eukaryotic genomes • Differences in codon usage between organisms • Eukaryotic genes containing introns will not be expressed properly in prokaryotes