思考基因编辑+抗菌肽？ 特a学 Transformation Appropriate in bacteria cloning vector Recombinant expression CRISPR-Cas9. of AMP DTMA Transformation in yeast DNA sequence Target containing gene Cloned gene for AMP encoding vector Double AMP Transformation Transgenic stranded in plants expression @出▣ DNA of AMP break 岗H Figure 1 Dg Dacovery br Integrating CRISPR-Cas9 based gene editing with existing genetic engineering approaches for production of novel antimicrobial peptides AMPs 7 AMPs-gRNA gRNA Cas9 Mammal cells In vitro transcription Electroporation 约 基因编辑手段构建、改造 Protein Pept Lett.2019:26(2):79-87.思考 基因编辑 + 抗菌肽？ 基因编辑手段构建、改造 严禁复制