No of Pages 9 06 September 2019 ARTICLE IN PRESS Cheng Wang et al. Neuroscience 418(2019)XXx-XXX Ventral CA1 口Fema|e AP3.4 500AP-34 Naive EPM F Basolateral amygdala -Ez 50000 AP-1.6 P-1.6 Naive EPM I Prelimbic cortex 864 Naive EPM Fig. 7. Stronger enrollment of ventral hippocampus in innate anxiety in male mice.(A, B)Representative images of vCA1(AP-3. 4 mm relative to Bregma) C-Fos expression in males(A)and females(B)after exploration in an EPM (C)Increased c-Fos expression in the vCAl of both sexes after EPM explora tion, especially in males.(D, E)Representative images depicting quantification of basolateral amygdala(AP-1.6 mm relative to Bregma)c-Fos expres sion in males and females after the elevated plu test. (F) Increased c-Fos expression in the basolateral amygdala of both sexes after EPM exploration, with similar expression levels between males and females. (G, H)Representative images of prelimbic cortex(AP 1.8 mm relative to Bregma) C-Fos expression in males and females after the elevated plus-maze test. ()Increased c-Fos expression in the prelimbic cortex of both sexes after EPM xploration, with similar expression levels between males and females. Scale bar: 100 um. "P<.05, "P<. 01, ""P<. 001, two-way ANOVA with Bon ferroni's test, 3 mice/group, 3 slices/mouse EPM: elevated plus-maze (Cenquizca and Swanson, 2007). However, most of these ng Natural Science Foundation(5182013)and"111"Pro- studies are performed in male animals. The present study of Ministry of Education of the People's Republic of China did not dissect specific hippocampal subpopulations or cir cuits in anxiety modulation, leaving it an open question for CONFLICT OF INTEREST further research The authors declare no competing financial interests ACKNOWLEDGMENTS REFERENCES This study was supported by grants from the Ministry of Science and Technology of China(2014CB548200 and Adhikari A, Topiwala MA, Gordon JA.(2010)Synchronized activity 2015CB554503), National Natural Science Foundation of between the ventral hippocampus and the medial prefrontal cortex duringanxietyNeuron65(2):257-269,https://doiorg/10.1016/j China(31872774,91732107,81571067and81521063)(Cenquizca and Swanson, 2007). However, most of these studies are performed in male animals. The present study did not dissect specific hippocampal subpopulations or cir￾cuits in anxiety modulation, leaving it an open question for further research. ACKNOWLEDGMENTS This study was supported by grants from the Ministry of Science and Technology of China (2014CB548200 and 2015CB554503), National Natural Science Foundation of China (31872774, 91732107, 81571067 and 81521063), Beijing Natural Science Foundation (5182013) and “111” Pro￾ject of Ministry of Education of the People's Republic of China. CONFLICT OF INTEREST The authors declare no competing financial interests. REFERENCES Adhikari A, Topiwala MA, Gordon JA. (2010) Synchronized activity between the ventral hippocampus and the medial prefrontal cortex during anxiety. Neuron 65(2):257-269, https://doi.org/10.1016/j. neuron.2009.12.002. Fig. 7. Stronger enrollment of ventral hippocampus in innate anxiety in male mice. (A, B) Representative images of vCA1 (AP -3.4 mm relative to Bregma) c-Fos expression in males (A) and females (B) after exploration in an EPM. (C) Increased c-Fos expression in the vCA1 of both sexes after EPM explora￾tion, especially in males. (D, E) Representative images depicting quantification of basolateral amygdala (AP -1.6 mm relative to Bregma) c-Fos expres￾sion in males and females after the elevated plus-maze test. (F) Increased c-Fos expression in the basolateral amygdala of both sexes after EPM exploration, with similar expression levels between males and females. (G, H) Representative images of prelimbic cortex (AP 1.8 mm relative to Bregma) c-Fos expression in males and females after the elevated plus-maze test. (I) Increased c-Fos expression in the prelimbic cortex of both sexes after EPM exploration, with similar expression levels between males and females. Scale bar: 100 μm. *P < .05, **P < .01, ***P < .001, two-way ANOVA with Bon￾ferroni's test, 3 mice/group, 3 slices/mouse. EPM: elevated plus-maze. Cheng Wang et al. / Neuroscience 418 (2019) xxx–xxx 7 NSC 19240 No of Pages 9 06 September 2019