中国组织工程研究第们7卷第41朝2013-10-08出版 Chinese Joumal of Tissue Engineering Research October 8, 2013 Vol 17, No 41 Cterwww.crter.OrG doi:10.3969/.issn.2095-4344.2013.41.023/http.//www.crter.org 傅重洋,赵佳,曲巍组织工程化神经修复周固神经创伤的应用中国组织工程研究,2013,1741)73357340 组织工程化神经修复周围神经创伤的应用☆ 傅重洋',赵佳2,曲巍(大连医科大学附属第一医院手显微外科,辽宁省大连市116011:2大连市第六人民医院CU病房 辽宁省大连市116031) 文章亮点 1此问题的已知信息:组织工程方法修复周围神经损伤已受到广泛关注,逐渐成为组织工程研究的焦点 重洋☆,男,1975年生 2文章增加的新信息:文章分类介绍了组织工程的3要素,又分别系统分析了各要素在周围神经损伤修复 省营口市人,汉族 方面的研究现状与进展 005年华中科技大学 3临床应用的意义:加快神经组织工程方面的应用研究,是促进组织工程从实验向临床转化的跨越。 济医学院毕业,博士,副 关键词 组织构建:组织构建综述:周围神经:神经损伤:干细胞:许旺细胞:间充顾干细胞:脂肪干细胞神经千觉手种经横伤与秀生的听 fuchongyang@ 主题词 hotmail. com 周围神经:神经损伤:干细胞:许旺细胞:生物相容性材料 中图分类号R318 文献标识码 文章编号:20954344 背景:近年来,随着生物工程技术以及组织工程化神经的发展给周围神经缺损的治疗带来了新的希望,已逐 (201341-0733506 渐成为研究的焦点 收槁日期:201307-05 目的:从种子细胞、生物材料以及构建周围神经组织技术3个方面综述组织工程方法修复周围神经损伤的新修曰目期:2013-08-23 进展。 (201307021M·W 方法:由第一作者在2013年7月应用计算机检索 PubMed数据库及CNK数据库,英文关键词为“ tissue engineering, peripheral nerves, nerve injuries, stem cells, schwann cells, scaffold, growth factor 中文关键词为“组织工程,周围神经,神经损伤,干细胞,许旺细胞,支架,生长因子”。选择内容与神经 组织工程、周围神经损伤修复相关的文章,同一领域文献则选择近期发表或发表在权威杂志文章,共纳入63 篇文献 结果与结论:现阶段组织工程方法修复周围神经损伤的研究虽已取得很大进展,但大多停留于实验探索阶段。 将组织工程神经应用于临床尚存下列问题亟待解决:①种子细胞来源及伦理。②细胞扩增后移植的免疫排斥 ③移植细胞稳定性问题及致瘤性。④神经支架材料的降解速度、最佳孔隙率、导管厚度、形状等。⑤体外神 经构建后移植修复时机。⑥各种神经生物因子的局部释放与调控等等。随着科技的发展,期待上述问题的解 决,从而使得众多临床神经损伤患者受益。 Tissue-engineered nerve for repair of peripheral nerve injuries Fu Chong-yang, Zhao Jia, Qu Wei(Department of Hand Surgery and Microsurgery, First Afiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China; ICU, Sixth Peoples Hospital of Dalian, Dalian 116031, Liaoning Province, China) BACKGROUND: Recent development of bioengineering technology and tissue-engineered nerve brings a new hope for the treatment of peripheral nerve injuries, which has gradually become a research spot. OBJECTIVE: To review the new progress in the repair of peripheral nerve injuries using seed cells, biomaterials and tissue-engineered nerve construction technology METHODS: PubMed and CNKI were searched by the first authors for articles concerning nerve tissue Fu Chong-yang☆,MD. ngineering and repair of peripheral nerve injuries published prior to July 2013. The keywords were tissue Associate chief physician. engineering, peripheral nerves, nerve injuries, stem cells, Schwann cells, scaffold, growth factor"in English and Associate professor. Chinese, respectively. The articles published recently or in the authorized journals were preferred in the same and Microsurgery field. Finally, 63 articles were included in result analysis filiated Hospital of Dalian ESULTS AND CONCLUSION: Up to now, there is a gre ance in the tissue engineering technology for the Medical University, Dalian repair of peripheral nerve injuries. However, most studies are still in experimental step. For the clinical application 16011, Liaoning Province, of nerve tissue engineering, some problems to be solved include: (1)source and ethics of seed cells; (2) immunological rejection following cell proliferation and transplantation; ( 3) stability and oncogenicity of fuchongyangghotmail.com transplanted cells; (4)degradation rate, optimal porosity, tube thickness and shape; (5) iming for in vitro sue-engineered nerve construction; (6)local release and regulation of various neurobiological factors. With the Received: 2013507-0 development of science, many patients with nerve injuries can profit from the solve of these problems Accepted: 2013-08-23 /SSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 7335 o1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp:/www.cnki.net中国组织工程研究 第 17 卷 第 41 期 2013–10–08 出版 Chinese Journal of Tissue Engineering Research October 8, 2013 Vol.17, No.41 doi:10.3969/j.issn.2095-4344.2013.41.023 [http://www.crter.org] 傅重洋，赵佳，曲巍. 组织工程化神经修复周围神经创伤的应用[J].中国组织工程研究，2013，17(41):7335-7340. ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 7335 www.CRTER.org 傅重洋☆，男，1975 年生， 辽宁省营口市人，汉族， 2005 年华中科技大学同 济医学院毕业，博士，副 主任医师，副教授，主要 从事神经损伤与再生的研 究。 fuchongyang@ hotmail.com 中图分类号:R318 文献标识码:A 文章编号:2095-4344 (2013)41-07335-06 收稿日期：2013-07-05 修回日期：2013-08-23 (201307021/M·W) Fu Chong-yang☆, M.D., Associate chief physician, Associate professor, Department of Hand Surgery and Microsurgery, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China fuchongyang@hotmail.com Received: 2013-07-05 Accepted: 2013-08-23 组织工程化神经修复周围神经创伤的应用☆ 傅重洋 1 ，赵 佳 2 ，曲 巍 1 (1大连医科大学附属第一医院手显微外科，辽宁省大连市 116011；2大连市第六人民医院 ICU 病房， 辽宁省大连市 116031) 文章亮点： 1 此问题的已知信息：组织工程方法修复周围神经损伤已受到广泛关注，逐渐成为组织工程研究的焦点。 2 文章增加的新信息：文章分类介绍了组织工程的 3 要素，又分别系统分析了各要素在周围神经损伤修复 方面的研究现状与进展。 3 临床应用的意义：加快神经组织工程方面的应用研究，是促进组织工程从实验向临床转化的跨越。 关键词： 组织构建；组织构建综述；周围神经；神经损伤；干细胞；许旺细胞；间充质干细胞；脂肪干细胞；神经干 细胞；胚胎干细胞；支架；生长因子 主题词： 周围神经；神经损伤；干细胞；许旺细胞；生物相容性材料 摘要 背景：近年来，随着生物工程技术以及组织工程化神经的发展给周围神经缺损的治疗带来了新的希望，已逐 渐成为研究的焦点。 目的：从种子细胞、生物材料以及构建周围神经组织技术 3 个方面综述组织工程方法修复周围神经损伤的新 进展。 方法：由第一作者在 2013 年 7 月应用计算机检索 PubMed 数据库及 CNKI 数据库，英文关键词为“tissue engineering，peripheral nerves，nerve injuries，stem cells，schwann cells，scaffold，growth factor”， 中文关键词为“组织工程，周围神经，神经损伤，干细胞，许旺细胞，支架，生长因子”。选择内容与神经 组织工程、周围神经损伤修复相关的文章，同一领域文献则选择近期发表或发表在权威杂志文章，共纳入 63 篇文献。 结果与结论：现阶段组织工程方法修复周围神经损伤的研究虽已取得很大进展，但大多停留于实验探索阶段。 将组织工程神经应用于临床尚存下列问题亟待解决：①种子细胞来源及伦理。②细胞扩增后移植的免疫排斥。 ③移植细胞稳定性问题及致瘤性。④神经支架材料的降解速度、最佳孔隙率、导管厚度、形状等。⑤体外神 经构建后移植修复时机。⑥各种神经生物因子的局部释放与调控等等。随着科技的发展，期待上述问题的解 决，从而使得众多临床神经损伤患者受益。 Tissue-engineered nerve for repair of peripheral nerve injuries Fu Chong-yang1 , Zhao Jia2 , Qu Wei1 (1 Department of Hand Surgery and Microsurgery, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China; 2 ICU, Sixth People’s Hospital of Dalian, Dalian 116031, Liaoning Province, China) Abstract BACKGROUND: Recent development of bioengineering technology and tissue-engineered nerve brings a new hope for the treatment of peripheral nerve injuries, which has gradually become a research spot. OBJECTIVE: To review the new progress in the repair of peripheral nerve injuries using seed cells, biomaterials and tissue-engineered nerve construction technology. METHODS: PubMed and CNKI were searched by the first authors for articles concerning nerve tissue engineering and repair of peripheral nerve injuries published prior to July 2013. The keywords were “tissue engineering, peripheral nerves, nerve injuries, stem cells, Schwann cells, scaffold, growth factor” in English and Chinese, respectively. The articles published recently or in the authorized journals were preferred in the same field. Finally, 63 articles were included in result analysis. RESULTS AND CONCLUSION: Up to now, there is a great advance in the tissue engineering technology for the repair of peripheral nerve injuries. However, most studies are still in experimental step. For the clinical application of nerve tissue engineering, some problems to be solved include: (1) source and ethics of seed cells; (2) immunological rejection following cell proliferation and transplantation; (3) stability and oncogenicity of transplanted cells; (4) degradation rate, optimal porosity, tube thickness and shape; (5) repair timing for in vitro tissue-engineered nerve construction; (6) local release and regulation of various neurobiological factors. With the development of science, many patients with nerve injuries can profit from the solve of these problems