BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 silaffin peptide in grooves of gel surface . emea Reverse recognition: Using synthetic inorganic materials to quide localization of biological targets" 回 Figure 4 Phage recognition of semiconductor heterostructures a-C, Fuorescence mages related to GaAs recognition by phage a, Control exper iment: no phage is present, but primary antibody and streptavidin-tetramethryl rhodamine(MR) are present b, The GaAs clone G12-3 was interacted with a substrate patterned with 1-um GaAs lines and 4-um SiO, spaces. The phage were then fluorescently labelled with TMR. The G12-3 cone specically recognzed the GaAs and not the siO, surface; scale bar, 4 um. A diagram of this recognition process is shown in d, in which phage specifically attach to one semiconductor rather than another. in astructure. C, An SEM image of a heterostructure containing altemating layers of GaAs and AlagdGao a As, used to demonstrate that this recognition is element-specific. The cleaved with G12-3 phage, and the phage was then tagged with 20-nm nanoparticles (shown arrowed in c) are located on GaAs and nota 500nm. e, Diagram illustrating the use of this specificity to desig heterostructures using proteins with multiple recognition sites. (Belcher lab) Lecture 12-Inorganic Biomaterials 8of13BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 + Si(OH)4 (aq) [10 min. room temp] Silaffin peptide in grooves of gel surface Reverse recognition: Using synthetic inorganic materials to guide localization of biological targets4 (Belcher lab) Lecture 12 – Inorganic Biomaterials 8 of 13