BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 salyze Recognitive Prole Lectins fe Biemdlewle iie amino aed residues Biomolecule imvolved in specifie recognition) t Choos or Desien Reooemtive Polymer Syntess Resdue functional t D Wash 8武一 二冒生品 types of target molecules: 1 o small-molecule drugs o steroids o nucleic acids o amino acids o metal ions o proteins Structure of Molecularly-Imprinted Networks structure of molecularly-imprinted networks o imprinted networks can be confined to a thin surface layer or prepared in bulk o surface networks usually perform better for capture of large molecules like proteins simple synthetic components for recognition networks o monomers o itaconic acid o acrylamides 4-vinyl pyrrolidone o other designed mo cross-linker o ethylene glycol dimethacrylate o PEG dimethacrylate o 'chain effect o binding of monomers to macromolecular templates causes a reduction in chain termination and thus an overall increase in reaction rate Example of molecular recognition: molecular imprinting of D-glucose(Peppas) o Monomers chosen as analogs of the amino acid residues that bind to glucose in vivo O WHAT RECEPTORS BIND GLUCOSE? Aspartate Lecture 10-Bioengineering Applications of Hydrogels 20f12BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 • types of target molecules: 1 o small-molecule drugs o steroids o nucleic acids o amino acids o metal ions o proteins Structure of Molecularly-Imprinted Networks • structure of molecularly-imprinted networks o imprinted networks can be confined to a thin surface layer or prepared in bulk o surface networks usually perform better for capture of large molecules like proteins • simple synthetic components for recognition networks o monomers: o methacrylic acid o itaconic acid o acrylamides o 4-vinyl pyrrolidone o β-cyclodextrin o other designed monomers o cross-linkers o ethylene glycol dimethacrylate o PEG dimethacrylate o ‘chain effect’3 o binding of monomers to macromolecular templates causes a reduction in chain termination and thus an overall increase in reaction rate • Example of molecular recognition: molecular imprinting of D-glucose (Peppas) o Monomers chosen as analogs of the amino acid residues that bind to glucose in vivo: o WHAT RECEPTORS BIND GLUCOSE? • Aspartate Lecture 10 – Bioengineering Applications of Hydrogels 2 of 12