BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 o Draw structures on board o Simple synthetic monomers chosen to mimic the bonding interactions of these amino acids Hydroxyethyl methacrylate CH CH- OCH Acrylic acid TARGET: D-glucose Specificity of binding sues. 0.8 Tightly cross-linked networks hold functional group positions for better Bound 0 45 mu bound g cry polymer recognition but restrict entry of target into network 30 40 50 60 Limited complexity in recognition units Time(hrs) c时出 copolymers6 C贴 Mnomn.Fayme i wear:Ar把过用20 MA Copolymers wth67% Competive substrate pntd intensiy Nonimprinted intensity 3±11.81 5097±07 81±1383585 Flores Fluorescent analogue 5657±090 Improving recognition by surface templating(Ratner+5) o Protein adsorbed to mica surface, coated with disaccharide, then coated with C3 Fs film by radiofrequency glow-discharge plasma treatment o Sugar coating protects protein from denaturation on dehydration Lecture 10-Bioengineering Applications of Hydrogels 3of12BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 • Glutamate • Asparagines • Serine o Draw structures on board o Simple synthetic monomers chosen to mimic the bonding interactions of these amino acids: QuickTime™ and a Graphics decompressor are needed to see this picture. TARGET: D-glucose Hydroxyethyl methacrylate Acrylic acid acrylamide Specificity of binding: Issues: Tightly cross-linked networks hold functional group positions for better recognition but restrict entry of target into network Limited complexity in recognition units • Improving recognition by surface templating (Ratner 4,5) o Protein adsorbed to mica surface, coated with disaccharide, then coated with C3F6 film by radiofrequency glow-discharge plasma treatment o Sugar coating protects protein from denaturation on dehydration Lecture 10 – Bioengineering Applications of Hydrogels 3 of 12