Yano et al Page 16 n s ns 火★★ 125 2.0 0.75 0.25 9 0.00 P0P21P42P42 SPF GF P0 P21 P42 P0 P21 P42 P42 GF+CONV. SPF+ABX VEH GF+CoNV. SPF+ABX VEH ns ns C D z∝E.<g SPF GF P0 P21 P42 P0 P21 P42P42 SPF GF P0 P21 P42 P0 P21 P42 P42 GF+CoNV. SPF+ABX VEH iF+CONV. SPF+ABX VEH Figure 1. The Gut Microbiota Modulates Host Peripheral Serotonin Levels A)Levels of serum 5-HT. Data are normalized to serum 5-HT in SPF mice. n=8-13 (B)Levels of colon 5-HT relative to total protein. Data are normalized to colon 5-HT elative to total protein in SPF mice. n=8-13 (C)Colonic expression of TPHI relative to GAPDH. Data are normalized to expression (D)Colonic expression of SLC6A4 relative to GAPl Data are normalized to expression levels in SPF mice. n=4. Data are presented as mean+ SEM.'p<0.05,**p<0.01, ***p<0.001, n.s. =not statistically significant. SPF=specific Cell. Author manuscript; available in PMC 2016 April 09Figure 1. The Gut Microbiota Modulates Host Peripheral Serotonin Levels (A) Levels of serum 5-HT. Data are normalized to serum 5-HT in SPF mice. n=8–13. (B) Levels of colon 5-HT relative to total protein. Data are normalized to colon 5-HT relative to total protein in SPF mice. n=8–13. (C) Colonic expression of TPH1 relative to GAPDH. Data are normalized to expression levels in SPF mice. n=4. (D) Colonic expression of SLC6A4 relative to GAPDH. Data are normalized to expression levels in SPF mice. n=4. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n.s.=not statistically significant. SPF=specific Yano et al. Page 16 Cell. Author manuscript; available in PMC 2016 April 09. Author Manuscript Author Manuscript Author Manuscript Author Manuscript