by comparing the purified antibody activity to the starting material in a series of titrations.There are a wide-variety of antibody titration procedures.A few of the most common are:precipitation,agglutination, complement fixation,ELISA,and RIA.Some activity of the antibody preparation is generally lost during purification but the specific activity will increase (Figure 26.). Protein purification procedures Purification and characterization is a prerequisite to studying all biological molecules and antibodies are no exception.The first step in characterizing antibodies is isolation of the molecule in pure form.There are many procedures but only those most commonly used for antibodies will be briefly discussed.Each of these procedures will be covered in greater detail in other lectures in this Biotechnology Seminar.Most of these procedures can be automated and improved by HPLC technology.Most HPLC versions of the procedures will bring about a reduction in purification time,increased resolution and better data acquisition. Figure 26 by comparing the purified antibody activity to the starting material in a series of titrations. There are a wide-variety of antibody titration procedures. A few of the most common are: precipitation, agglutination, complement fixation, ELISA, and RIA. Some activity of the antibody preparation is generally lost during purification but the specific activity will increase (Figure 26.). Protein purification procedures Purification and characterization is a prerequisite to studying all biological molecules and antibodies are no exception. The first step in characterizing antibodies is isolation of the molecule in pure form. There are many procedures but only those most commonly used for antibodies will be briefly discussed. Each of these procedures will be covered in greater detail in other lectures in this Biotechnology Seminar. Most of these procedures can be automated and improved by HPLC technology. Most HPLC versions of the procedures will bring about a reduction in purification time, increased resolution and better data acquisition. Figure 26