11.7 Shuttle Vectors and Expression Vectors Shuttle vecfors:vectors that are stably maintained in two or more unrelated host organisms (e.g.,E.coli and B. subtilis or E.coli and yeast)穿梭载体 Bacterial plasmid engineered to function in eukaryotes Add a eukaryotic origin of replication Add a centromere recognition sequence Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology 11.7 Shuttle Vectors and Expression Vectors Shuttle vectors: vectors that are stably maintained in two or more unrelated host organisms (e.g., E. coli and B. subtilis or E. coli and yeast) 穿梭载体 • Bacterial plasmid engineered to function in eukaryotes • Add a eukaryotic origin of replication • Add a centromere recognition sequence