The Na+-K+ATPase CUTSIDE Oligosaccharides CF CELL The Na+-K+ATPase requires K+outside,Nat and ATP inside,and is inhibited by ouabain. INSIDE OE CELL Binding ste for ATP The ratio of Na+:K+pumped is 3:2 for each ATP Figure 8-10 The Na/K+Pump.The Na'/K+pump found in most animal cells consists of two and two subunits.The subunits are hydrolyzed. transmembrane proteins,with binding sites for ATP on the cytoplasmic side.The B subunits are located on the outer side of the membrane and are glycosylated.The pump is shown in the E conformation,which is The Na+-K+ATPase is a P- open to the inside of the cell.Binding of sodium ions causes a conformational change to theE form,which opens to the outside. type pump.This ATPase ouabain- 36 sequentially phosphorylates binding site and dephosphorylates itself electrochemical during the pumping cycle. gradient CYTOSOL The Na+-K+ATPase is found only in animals. ATP ADP This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information