Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S Gerstein opyright◎2001 ISBNS:0-471-37972-7( Paper);0-47 (Electronic) How to Properly Use and Maintain Laboratory Equipment Trevor Troutman Kristin A. Prasauckas Michele A Kennedy, Jane Stevens, Michael G. Davies, and Andrew T Dadd Balances and scales 5 How Are Balances and scales Characterized? How Can the Characteristics of a Sample and the Immediate Environment Affect Weighing Reproducibility? 5 By What Criteria Could You Select a Weighing Instrument? How Can You generate the most reliable and Reproducible Measurements? How Can You minimize service calls 55 Centrifugation Theory and Strategy Practice Centrifugation of DNA and rNa 63 Troubleshooting Pipettor Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc.49 4 How to Properly Use and Maintain Laboratory Equipment Trevor Troutman, Kristin A. Prasauckas, Michele A. Kennedy, Jane Stevens, Michael G. Davies, and Andrew T. Dadd Balances and Scales . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 How Are Balances and Scales Characterized? . . . . . . . . . . . . 51 How Can the Characteristics of a Sample and the Immediate Environment Affect Weighing Reproducibility? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 By What Criteria Could You Select a Weighing Instrument? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 How Can You Generate the Most Reliable and Reproducible Measurements? . . . . . . . . . . . . . . . . . . . . . . . 54 How Can You Minimize Service Calls? . . . . . . . . . . . . . . . . . . 55 Centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Theory and Strategy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Practice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Centrifugation of DNA and RNA . . . . . . . . . . . . . . . . . . . . . . 63 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Pipettors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc. Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic)