武汉大学生命科学学院 2003-2004学年第一学期期末考试 《分子生物学》试卷及参考答案 Final exam of molecular Biology Course(spring 2004) 写在参考答案前面的话 该课程考试目的是考査学生对所学知识掌握的情况,除选择题外,其他题目的答 案基本都不是唯一的。你可以从不同的角度去阐明一个概念。 公布参考答案的目的:为该课程画个句号,让学生过个安心暑假 Best wishes to all of you PART I: DESCRIPtion (2 points each) Your answer should describe what each item is and how it functions in the cell. diagrams structure and sequence information should be included in your answer, as necessary 1. Yeast artificial chromosome: ①酵母人工染色体_(05 point.) 2 contains components required for replication and segregation of the natural yeast chromosome, includ ing two telomeric sequences(TEL), one centromere(CEN and one autonomously replicating sequences(ARs)(0.75 point contains genes act as selective markers in yeast and proper restriction sites(0.75 4 can accommodate genomic DNA fragments of more than 1 Mb(0.5 point) 2. RNA interference ①RNA干扰_(O5 point) 2 a conserved biological response to double-stranded rNa(I point) 3 regulates the expression of protein-cod ing genes through siRNA or miRNA( DoIn a the dsRNa is restricted by DICER, then RISC med iates the siRNa and miRNA related rna degradation and translation inhibition, respectively. (I point) 3. Proteomics ①蛋白组学(O5 point) The study of the proteome using techniques of high resolution protein separation and identification (1. 5 point) 4 The best separation method is two dimensional gel electrophoresis, the individual protein spots are then cut from the gel and treated with protease to produce a set of peptides characteristic of that protein. The precise masses of each peptide in the sample are then determined by MAlDI mass spectrometry. The resulted peptide mass fingerprint of that protein is then compared to a database to deduce the function of that protein etc. (1.5 point) 4. Shine-Dalgarno sequence ①SD序列(O5poin) 2 A conserved sequence 8-13 nt upstream of the first codon to be translated(1 This sequence was discovered by Shine and Dalgarno(0.5 point) 4 The sequence is purine-rich and contains all or part of5'-AGGAGGU-3'(0.5武汉大学生命科学学院 2003－2004 学年第一学期期末考试 《分子生物学》试卷及参考答案 Final exam of Molecular Biology Course (Spring 2004) 写在参考答案前面的话： ➢ 该课程考试目的是考查学生对所学知识掌握的情况，除选择题外，其他题目的答 案基本都不是唯一的。你可以从不同的角度去阐明一个概念。 ➢ 公布参考答案的目的：为该课程画个句号，让学生过个安心暑假 ➢ Best wishes to all of you PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. 1. Yeast artificial chromosome: ① 酵母人工染色体（0.5 point） ② contains components required for replication and segregation of the natural yeast chromosome, including two telomeric sequences (TEL), one centromere (CEN) and one autonomously replicating sequences (ARS) (0.75 point ) ③ contains genes act as selective markers in yeast and proper restriction sites (0.75 point ) ④ can accommodate genomic DNA fragments of more than 1 Mb (0.5 point) 2. RNA interference: ① RNA 干扰 （0.5 point） ② a conserved biological response to double-stranded RNA (1 point) ③ regulates the expression of protein-coding genes through siRNA or miRNA (1 point) ④ the dsRNA is restricted by DICER, then RISC mediatesthe siRNA and miRNA related RNA degradation and translation inhibition, respectively. (1 point) 3. Proteomics ① 蛋白组学 （0.5 point）The study of the proteome using techniques of high resolution protein separation and identification (1.5 point) ④ The best separation method is two dimensional gel electrophoresis, the individual protein spots are then cut from the gel and treated with protease to produce a set of peptides characteristic of that protein. The precise masses of each peptide in the sample are then determined by MALDI mass spectrometry. The resulted peptide mass fingerprint of that protein is then compared to a database to deduce the function of that protein etc. (1.5 point) 4. Shine-Dalgarno sequence ① SD 序列 （0.5 point） ② A conserved sequence 8-13 nt upstream of the first codon to be translated (1 point). ③ This sequence was discovered by Shine and Dalgarno (0.5 point) ④ The sequence is purine-rich and contains all or part of 5’-AGGAGGU-3’ (0.5