FIGURE 1 plate 1 plate 2 plate 3 minimal =edium + phe plate 4 plate 5 a)Plate 1 is the original rich medium plate that the mutagenized cells were grown on. It is standard practice for the last plate in a series of replica plates to have the same growth medium as the original plate. What purpose might this serve? It is a control. You want to make sure that cells from each colony were transferred to all previous plates so you can assess growth versus no growth b)You obtain some phe- colonies in this manner. Identify the colonies from FIGURE 1 that are phe CG.EHY c)You notice that some colonies do not grow on plates containing minimal medium phenylalanine(plate 2). Identify these colonies and give one possible explanation for the growth behavior of these colonies Cells from colonies B, D, and X may have a mutation in a gene that is important for the synthesis of any compound other than phenylalanine d)This strategy for isolating phe" mutants works well so you repeat the mutagenesis and replica plating experiment to isolate more phe" mutants. You then perform a complementation test on these phe"mutants i)What is a complementation test? A complementation test consists of creating a diploid that carries two independently isolated mutations and examining if the diploid has a mutant or a wildtype phenotype genes. If the diploid has a mutant phenotype, then one assumes that the mutations are in the ?s If the diploid has a wildtype phenotype, then one assumes that the two mutations are in differe same gene ii)What is the purpose of a complementation test? A complementation test can identify how many different genes are represented in a group of utants 012Fal20037.012 Fall 2003 FIGURE 1: A B C D E F G H X Y A F A B F A C E F G H Y A B C D E F G H X Y plate 1 plate 2 plate 3 plate 4 plate 5 rich medium minimal medium + phe minimal medium minimal medium + PEP rich medium a) Plate 1 is the original rich medium plate that the mutagenized cells were grown on. It is standard practice for the last plate in a series of replica plates to have the same growth medium as the original plate. What purpose might this serve? It is a control. You want to make sure that cells from each colony were transferred to all previous plates so you can assess growth versus no growth. b) You obtain some phe- colonies in this manner. Identify the colonies from FIGURE 1 that are phe￾C,G,E,H,Y c) You notice that some colonies do not grow on plates containing minimal medium + phenylalanine (plate 2). Identify these colonies and give one possible explanation for the growth behavior of these colonies. Cells from colonies B, D, and X may have a mutation in a gene that is important for the synthesis of any compound other than phenylalanine. d) This strategy for isolating phe- mutants works well so you repeat the mutagenesis and replica plating experiment to isolate more phe- mutants. You then perform a complementation test on these phe- mutants. i) What is a complementation test? A complementation test consists of creating a diploid that carries two independently isolated mutations and examining if the diploid has a mutant or a wildtype phenotype. If the diploid has a wildtype phenotype, then one assumes that the two mutations are in different genes. If the diploid has a mutant phenotype, then one assumes that the mutations are in the same gene. ii) What is the purpose of a complementation test? A complementation test can identify how many different genes are represented in a group of mutants