Question 2, continued In the table below, a(+)indicates that the diploid created grows on minimal media, a(-) indicates that the diploid fails to grow on minimal media m2 m3 9 m10 12 + ++ ++ + 14 me 5678 十十+ ++ + 十+++-+++- +++++十+ + m10+ i)Assign the mutants 1-10 into complementation groups m1,m2,m7 14,m10 m6,m8 e) You also characterize your mutants based upon which of the intermediates accumulates m2. accumulates x ccumulates z m4: accumulates pep m5: accumulates y m6: accumulates w Based on the above data, you predict that i) m1 has a mutation in gene-3 ii) m2 has a mutation in gene_ 3 m3 has a mutation in gene 5 m4 has a mutation in ge v)m5 has a mutatis 012Fal20037.012 Fall 2003 Question 2, continued In the table below, a (+) indicates that the diploid created grows on minimal media, a (-) indicates that the diploid fails to grow on minimal media. m1 m2 m3 m4 m5 m6 m7 m8 m9 m10 m1 - - + + + + - +++ m2 - - + + + + - +++ m3 + + - + + + + +++ m4 + + + - + + + ++- m5 + + + + - + + +- + m6 + + + + + - + - ++ m7 - - + + + + - +++ m8 + + + + + - + - ++ m9 + + + + - + + +- + m10 + + + - + + + ++- ii) Assign the mutants 1-10 into complementation groups. m1, m2,m7 m3 m4,m10 m5, m9 m6, m8 e) You also characterize your mutants based upon which of the intermediates accumulates m2: accumulates X m3: accumulates Z m4: accumulates PEP m5: accumulates Y m6: accumulates W Based on the above data, you predict that i) m1 has a mutation in gene___3___ ii) m2 has a mutation in gene___3___ iii) m3 has a mutation in gene___5___ iv) m4 has a mutation in gene___1___ v) m5 has a mutation in gene___4___