BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 The soace betweer the kinesin ain to prevent nonspecific surface adsorpton of the indicate the paths af indvidual mcrotwbu (Vogel, 2002) We don't yet understand the physicochemical principles controlling molecular motor speed, unloading/loading of cargo A molecular rotor built from atPase Question: how do we create engines to provide piconewton forces for nanodevices? o One answer: engineered materials based on protein motor-based engines Work of the group of Carlo Montemagno at UCLA (Dept of Bioengineering) o Role of this protein in cell ATPase complex No-load rotational velocity of -17 revolutions per second o Generates >80 pNnm work o Approximately 100% efficient! o -10 nm diameter Lecture 13-Hybrid macromolecules 9 of 13BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 (Vogel, 2002) ƒ We don’t yet understand the physicochemical principles controlling molecular motor speed, unloading/loading of cargo A molecular rotor built from ATPase ƒ Question: how do we create engines to provide piconewton forces for nanodevices? o One answer: engineered materials based on protein motor-based engines ƒ Work of the group of Carlo Montemagno at UCLA (Dept. of Bioengineering)9-13 ƒ F1 fragment of adenosine triphosphate synthase (F1-ATPase) o Role of this protein in cell o Rotary motion during ATP hydrolysis as J subunit transitions between 3 equidistant positions around the ATPase complex ƒ No-load rotational velocity of ~17 revolutions per second o Generates > 80 pN•nm work o Approximately 100% efficient! o ~10 nm diameter Lecture 13 – Hybrid macromolecules 9 of 13