水产科学 第23卷 保水产品中无药物残留。在本实验条件下,建议罗1940 非鱼的停药期为22d,中国对虾为12d [5]胡功政,冯淇辉.恩诺沙星及其活性代谢物在鸡体内 的药物动力学[.中国兽医学报,1999,19(2):171 参考文献 l李家乐,李思发.中国大陆尼罗罗非鱼引进及研究进6】 Cinquin A L, Roberti F, Giannetti,ed. Determination of 展[]水产学报,2001,25(1):90-95 enrofloxacin and its metabolite ciprofloxacin in gpat milk by [2 Nitesh K, Singh S D, Jayachandran C Pharmacokinetics of highperformance liquid chromatography with diode array de Enrofloxacin and Its Active metabolite floxacin and Its tection Optimization and validation J]. Journal g Chromatog Interaction with diclofenac after Intravenous administration raphy 4,2003,987221-226 in Buffalo Calves[J]. The Veterinary Journal, 2003, 165 [7 Barron D JimenezLoza E, Cam J, et al. Determination of residues of enrofloxacin and its metabolite ciprofloxacin in br B3 Kung K, Pond J L, Wanner M Pharmacokinetics of Err logical materials by capillary electrophoresis[J]-Joumal d rofloxacin and Its Metabolite Ciprofloxacin after intravenous Chromatograp/y B,2001,759:73-79 and oral administration of Enrofloxacin in dogs [J].Jver8]祝万菊邓旭明,张艳萍等 沙星缓释溶液在猪 Phanmnco Therap, 1993, 16: 462-468 体内的药物动力学及生物利用度[].吉林农业大学学 [4 Brome RL, Brooks D L Pharmacokinetics properities of Er 报200325(3):335-338 rofloxacin in rabbits[J]. Am J Vet, 1992, 52(10): 1935 [9]庄无忌.各国食品和饲料中农药、兽药残留限量大全 [M]北京中国对外贸易出版社,1995 Residues of Enrofloxacin and Its Meta bolite in Jifu Tilapia and Penaeus chinensis XU Werhai'3LIN Liming, ZHU Xiaorbin', WANG Xirrting', HUANG Xirping',KANG Xing-lun (1. Center of Biotechology, Institute of Oceaplogy, Chinese Academy of Science, Qingdao 266071 2 Food Laboratory of Qingdao Entry-Exit Inspection and Quarantine Bureau of P. R. China, Qingdao 266002, China; 3. Graduate School Academy of Science Beijing 100039. China Abstract: The residues of enrofloxacin and its metabolite in Jifu Tilapia and Penaeus chinensis were determined after a single oral administration at a dose of 50 mg/ kg for 7 days. The results showed that enrofloxacin was changed into ciprofloxacin in Tilapia and Penaeus chinensis. The concentration of enrofloxacin in muscle, liver and blood of tilapia were(3. 61 t1. 02)Hg/ g, (5.96 +2. 12)Hg/g, (1. 25 +0. 23)ug/mL, respectively. And the elimination half-life were 15.61 h, 16.83 h, 17 19 h, respectively. The maximal concentration of ciprofloxacin in muscle was(0. 22 t0. 06) Hg/g, and the elimination half-life was 67 3 h. The maximal concentration of enrofloxacin and ciprofloxacin in Penaeus chinensis were(1. 68+0. 41)ug/g, (0.066 +0. 03)ug/g, respectively, And the elimination half-life were 27.9 h, 33.6 h, respectively. The predicted withdrawal time was 22 d in Tilapia, and 12 d in Penaeus chinensis Key words Jifu Tilapia, Penaeus chinensis; enrofloxacin; ciprofloxacin; metabolite 责任编辑:小舟) 2 01994-2007 China Academic Journal Electronie Publishing House. All rights reserved hp: /we cnki.net保水产品中无药物残留。在本实验条件下 , 建议罗 非鱼的停药期为 22 d , 中国对虾为 12 d。 参考文献 : [1 ] 李家乐 ,李思发 1 中国大陆尼罗罗非鱼引进及研究进 展[J ]1 水产学报 ,2001 ,25(1) :90 - 951 [2 ] Nitesh K, Singh S D , Jayachandran C1Pharmacokinetics of Enrofloxacin and Its Active Metabolite Ciprofloxacin and Its Interaction With Diclofenac After Intravenous Administration in Buffalo Calves [J ]1 The Veterinary Journal , 2003 ,165 : 302 - 3061 [3 ] Kung K, Piond J L , Wanner M1 Pharmacokinetics of En2 rofloxacin and Its Metabolite Ciprofloxacin after intraverous and oral administration of Enrofloxacin in dogs [J ]1J Vet Pharmcol Therap , 1993 ,16 :462 - 4681 [4 ] Brome R L ,Brooks D L1Pharmacokinetics properities of En2 rofloxacin in rabbits [J ]1Am J Vet , 1992 ,52 (10) : 1935 - 19401 [5 ] 胡功政 ,冯淇辉 1 恩诺沙星及其活性代谢物在鸡体内 的药物动力学[J ]1 中国兽医学报 , 1999 ,19 (2) :171 - 1741 [6 ] Cinquina A L ,Roberti P , Giannetti F , et al1Determination of enrofloxacin and its metabolite ciprofloxacin in goat milk by high2performance liquid chromatography with diode2array de2 tection Optimization and validation[J ]1Journal of Chromatog2 raphy A ,2003 ,987 :221 - 2261 [7 ] Barron D ,Jimenez2Lozano E ,Cano J , et al1 Determination of residues of enrofloxacin and its metabolite ciprofloxacin in bi2 ological materials by capillary electrophoresis[J ]1Journal of Chromatography B ,2001 ,759 :73 - 791 [8 ] 祝万菊 ,邓旭明 ,张艳萍 ,等 1 恩诺沙星缓释溶液在猪 体内的药物动力学及生物利用度[J ]1 吉林农业大学学 报 ,2003 ,25(3) :335 - 3381 [9 ] 庄无忌 1 各国食品和饲料中农药、兽药残留限量大全 [M]1 北京 :中国对外贸易出版社 ,19951 Residues of Enrofloxacin and Its Metabolite in Jifu Tilapia and Penaeus chinensis XU Wei2hai1 ,3 ,LIN Li2ming2 , ZHU Xiao2bin1 , WANG Xin2ting1 ,HUANG Xin2ping1 , KANG Xing2lun1 (11Center of Biotechnology , Institute of Oceanology ,Chinese Academy of Science ,Qingdao 266071 ,China ;21Food Laboratory of Qingdao Entry2Exit Inspection and Quaranrine Bureau of P1R1China , Qingdao 266002 ,China ;31Graduate School , Chinese Academy of Science ,Beijing 100039 ,China) Abstract : The residues of enrofloxacin and its metabolite in Jifu Tilapia and Penaeus chinensis were determined after a single oral administration at a dose of 50 mg/ kg for 7 days1 The results showed that enrofloxacin was changed into ciprofloxacin in Tilapia and Penaeus chinensis1 The concentration of enrofloxacin in muscle , liver and blood of Tilapia were (3161 ±1102) μg/ g , (5196 ±2112) μg/ g , (1125 ±0123) μg/ mL , respectively1 And the elimination half2life were 15161 h ,16183 h ,17119 h , respectively1 The maximal concentration of ciprofloxacin in muscle was (0122 ±0106) μg/ g ,and the elimination half2life was 6713 h1 The maximal concentration of enrofloxacin and ciprofloxacin in Penaeus chinensis were (1168 ±0141) μg/ g ,(01066 ±0103)μg/ g , respectively ,And the elimination half2life were 2719 h ,3316 h , respectively1 The predicted withdrawal time was 22 d in Tilapia , and 12 d in Penaeus chinensis1 Key words :Jifu Tilapia ; Penaeus chinensis ; enrofloxacin ; ciprofloxacin ; metabolite (责任编辑 :小 舟) 8 水 产 科 学 第 23 卷 © 1994-2007 China Academic Journal Electronic Publishing House. 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