TCD50的测定(Reed- Muench) 稀释度CPE 观察值 累积值 比值 (DF 阳性阴性阳性阴性 10 8/8 0 24 0 24/24100 10 7/8 8753 16 16/1794 10 5/8 9/13 69 10-4 3/8 4 9 4/13 31 10-5 1/8 3578 16 1/17 6 10-6 0/8 24 0/240 X=log,DF at next dilution above 50%-50) ( at next dilution above 50%-% at next dilution below 50% =1(6950)(69-31)=0.5 1035稀释度的加入病毒液含1个TCD50TCID50的测定(Reed-Muench) 稀释度 (DF) CPE 观察值 累积值 比值 % 阳性 阴性 阳性 阴性 10-1 8/8 8 0 24 0 24/24 100 10-2 7/8 7 1 16 1 16/17 94 10-3 5/8 5 3 9 4 9/13 69 10-4 3/8 3 5 4 9 4/13 31 10-5 1/8 1 7 1 16 1/17 6 10-6 0/8 0 8 0 24 0/24 0 X = log10DF (% at next dilution above 50% - 50) (% at next dilution above 50% - % at next dilution below 50%) = 1 (69-50)/(69-31) = 0.5 10-3.5稀释度的加入病毒液含1个TCID50