人工粘性末端的连接3‘突出末端 5" GCATG 3 sph I G CTGCA 3 3 GTACG 3ACGTC G Pst I TdT dcTP TdT dGTP GCATGCCCCCC 3 G CTGCAGGGGGG 3 3 CCCCCCGTACG 3 GGGGGGACGTC G 退火 5 GCATGCCCCCC G CGCAGGGGGG C GGGGGGACGTC G CCCCCCGTACG 5 GCATGCCCCCC CTGCAGGGGGG C GGGGGGACGTC G CCCCCCGTACG 5 Klenow [4-DNA ligase PstI 5" GCATGCCCCCCTGCAG CGCAGGGGGGCATGO CGTACGGGGGGACGTC GACGTCCCCCCGTACG 5" GCATGCCCCCCTGCAG CTGCAGGGGGGCATGO CGTACGGGGGGACGTC GACGTCCCCCCGTACG人工粘性末端的连接 3‘突出末端 CTGCA 3' G G 3' ACGTC 5' GCATG 3' C 5' C 3' GTACG T4-DNA ligase TdT dCTP TdT dGTP GCATGCCCCCC 3' C 退火 Pst I Pst I C 3' CCCCCCGTACG CTGCAGGGGGG 3' G G 3' GGGGGGACGTC 5' 5' GCATGCCCCCC G C GGGGGGACGTC CTGCAGGGGGG C G CCCCCCGTACG 5' 5' GCATGCCCCCC G C GGGGGGACGTC CTGCAGGGGGG C G CCCCCCGTACG 5' 5' GCATGCCCCCCTGCAG CGTACGGGGGGACGTC CTGCAGGGGGGCATGC GACGTCCCCCCGTACG 5' 5' GCATGCCCCCCTGCAG CGTACGGGGGGACGTC CTGCAGGGGGGCATGC GACGTCCCCCCGTACG Klenow Pst I Sph I