Special NOESY experiments Filtered,edited NOE:based H Labeled on selection of NOEs from two Unlabeled protein peptide 13C molecules with unique labeling patterns. Only NOEs at the interface .Transferred NOE:based on 1).faster build-up of NOEs in large versus small molecules;2) Fast exchange 3)NOEs of koff bound state detected at resonance frequencies of free state Only NOEs from bound state 1616 Special NOESY experiments •Filtered, edited NOE: based on selection of NOEs from two molecules with unique labeling patterns. Unlabeled peptide Labeled protein Only NOEs at the interface Only NOEs from bound state H H H H kon koff •Transferred NOE: based on 1) faster build-up of NOEs in large versus small molecules; 2) Fast exchange 3) NOEs of bound state detected at resonance frequencies of free state