Factors affecting tolerance role of antigen Factors which Favor immune Favor tolerance affect response response >Physical form of Large,aggregated, soluble,aggregate-free, antigen complex molecules, simple small molecules, properly processed not processed >Route of injection > Subcutaneous or Oral or,sometimes, intramuscular intravenous Dose of antigen Optimal dose Very large or very small dose Factors affecting tolerance role of antigen Factors which affect response Favor immune response Favor tolerance ➢ Physical form of antigen ➢ Route of injection ➢ Dose of antigen ➢ Large, aggregated, complex molecules, properly processed ➢ Subcutaneous or intramuscular ➢ Optimal dose ➢ soluble, aggregate-free, simple small molecules, not processed ➢ Oral or, sometimes, intravenous ➢ Very large or very small dose