第二节蛋白质的大规模分离鉴定技术 一、蛋白质二维电泳-质谱技术 (一)蛋白质二维电泳技术2-DE First Dimension: Second Dimension: Denaturing isoelectric SDS polyacrylamide focusing in presence of urea.Nonidet NP-40 gel rod rebuffered gel electrophoresis in discontinuous gradient gel in vertical gel rod in SDS buffer sample pH 3 e PH 10 pH 10 IEF SDS-PAGE 田 Separation acc.to Separation acc.to Isoelectric Points (charge) Molecular Weight(mass)第二节 蛋白质的大规模分离鉴定技术 一、蛋白质二维电泳-质谱技术 (一)蛋白质二维电泳技术2-DE IEF SDS-PAGE