—Dry heat Direct flaming • example: Inoculation loop Hot-air sterilization • Baking in oven • Very effective method of sterilization, but requires temperature of 170ºC [ 340 ºF]for about 2 hr.—Dry heat Direct flaming • example: Inoculation loop Hot-air sterilization • Baking in oven • Very effective method of sterilization, but requires temperature of 170ºC [ 340 ºF]for about 2 hr