following precautions should be observed to prevent accidents nd fires There should be no puddles of liquid on the horizontal surfaces of the electrophoresis cell The area around the power supply and cell should be dry The area for at least 6 inches around the power supply and cell should be bare of clutter and other equipment. Clear space means any fire or accident can be more easily controlled What Are the Requirements for Safe Equipment in Good Working Order? The wires connecting the cell to the power supply must be in good condition, not worn or cracked, and the banana plugs and jacks must be in good condition, not corroded or worn. Broken or worn wires can cause rapid changes in resistance, slow elec trophoresis or a halt in the run. All cables and connectors must be inspected regularly for breaks and wear The banana plugs on the ends of the wires should be rer rom the power supply at the end of the run by pulling them straight out. Grasp the plug, not the wire. If pulled at an angle, the solder joint attaching the banana plugs to the wires can loosen and cause the loss of the electrical circuit. on the cell core electrode banana posts with flattened baskets do not make good contact with the banana jack in the cell lid, and should be replaced. The banana jacks(female part) in the cell lid should be inspected regularly to make sure there is no corrosion Before starting an electrophoresis run, dry any liquid on the horizontal surfaces of the cell, especially near the banana plugs and jacks. Any liquid on the horizontal surfaces of the cell can arc during the run, damaging the cell and stopping the electrophoresis. POLYACRYLAMIDE (PAGE GELS-BEFORE SELECTING A GEL: GETTING THE BEST RESULTS FOR YOUR PURPOSE Before choosing which gel to use, it is important to consider several questions, all of which can help you choose the gel will give you the best results for your purpose. The next graphs provide information on how to select a gel percentage or pore size, when to use SDS-PAGE and when native PAGE, what buffer system to use, which crosslinker to use, and degree of resolution needed 337following precautions should be observed to prevent accidents and fires. • There should be no puddles of liquid on the horizontal surfaces of the electrophoresis cell. • The area around the power supply and cell should be dry. • The area for at least 6 inches around the power supply and cell should be bare of clutter and other equipment. Clear space means any fire or accident can be more easily controlled. What Are the Requirements for Safe Equipment in Good Working Order? The wires connecting the cell to the power supply must be in good condition, not worn or cracked, and the banana plugs and jacks must be in good condition, not corroded or worn. Broken or worn wires can cause rapid changes in resistance, slow elec￾trophoresis or a halt in the run. All cables and connectors must be inspected regularly for breaks and wear. The banana plugs on the ends of the wires should be removed from the power supply at the end of the run by pulling them straight out. Grasp the plug, not the wire. If pulled at an angle, the solder joint attaching the banana plugs to the wires can loosen and cause the loss of the electrical circuit. On the cell core, electrode banana posts with flattened baskets do not make good contact with the banana jack in the cell lid, and should be replaced. The banana jacks (female part) in the cell lid should be inspected regularly to make sure there is no corrosion. Before starting an electrophoresis run, dry any liquid on the horizontal surfaces of the cell, especially near the banana plugs and jacks. Any liquid on the horizontal surfaces of the cell can arc during the run, damaging the cell and stopping the electrophoresis. POLYACRYLAMIDE (PAGE) GELS—BEFORE SELECTING A GEL: GETTING THE BEST RESULTS FOR YOUR PURPOSE Before choosing which gel to use, it is important to consider several questions, all of which can help you choose the gel that will give you the best results for your purpose. The next para￾graphs provide information on how to select a gel percentage or pore size, when to use SDS-PAGE and when native PAGE, what buffer system to use, which crosslinker to use, and degree of resolution needed. Electrophoresis 337